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Characterization of virus infectivity and cell‐free capsid assembly of SIVMneCL8
Author(s) -
Dooher Julia E.,
Pineda Mario Javier,
Overbaugh Julie,
Lingappa Jaisri R.
Publication year - 2004
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2004.00074.x
Subject(s) - capsid , infectivity , group specific antigen , virology , biology , virus , clone (java method) , simian immunodeficiency virus , lentivirus , microbiology and biotechnology , genetics , gene , viral disease
We have previously described a cell‐free system that reconstitutes immature capsid assembly of Gag polypeptides from viruses belonging to three major primate lentiviral lineages, including HIV‐1, HIV‐2 and SIVagm. Studies described here examine a member of the SIVmac/Mne lineage, SIVMneCL8, using assays for virus production and infectivity as well as cellular events in capsid formation. We report that SIVMneCL8, a molecular clone with properties typical of transmitted viral variants, is less infectious per unit p27 Gag than another member of the SIVmac/Mne lineage, SIVmac239. SIVMneCL8 Gag polypeptides are arrested at an early stage of capsid assembly in the cell‐free system. Additionally, SIVMneCL8 Gag polypeptides associate minimally with the host factor human HP68. This is the first report of a primate lentivirus that does not complete capsid assembly in the cell‐free system.