Premium
Penile vibratory stimulation in the marmoset monkey: a practical alternative to electro‐ejaculation, yielding ejaculates of enhanced quality
Author(s) -
Schneiders A.,
Sonksen J.,
Hodges J.K.
Publication year - 2004
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2004.00058.x
Subject(s) - marmoset , ejaculation , semen , semen collection , callithrix , electroejaculation , biology , sperm , semen quality , andrology , primate , sperm quality , anatomy , sperm motility , artificial insemination , medicine , endocrinology , neuroscience , pregnancy , paleontology , genetics
The availability of sufficient amounts of spermatozoa of high quality is one of the main limiting factors in reproductive research and development of reproductive technologies in marmoset monkeys ( Callithrix jacchus ). Penile vibrostimulation (PVS) has been successfully used in semen collection in the squirrel monkey but with poor success rate in the marmoset. We report here on an improved protocol for PVS with a success rate of almost 90%. Ejaculates obtained by PVS were of enhanced quality compared with those obtained by rectal probe electro‐ejaculation (RPE). PVS ejaculates contained on average three to fourfold higher numbers of total and motile spermatozoa. Assessment of sperm kinematics using computer‐assisted sperm analysis indicated that there are also functional differences between spermatozoa collected by PVS and RPE. Marmoset spermatozoa in samples obtained by RPE swim in a more convoluted manner compared with those obtained by PVS.