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Identification of primitive hematopoietic progenitor cells in the rhesus macaque
Author(s) -
Rosenzweig Michael,
Marks Douglas F.,
DeMaria Mary Ann,
Connole Michelle,
Johnson R. Paul
Publication year - 2001
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2001.300105.x
Subject(s) - cd34 , biology , bone marrow , rhesus macaque , progenitor cell , haematopoiesis , cd38 , population , immunology , stem cell , hematopoietic stem cell , microbiology and biotechnology , medicine , environmental health
The close phylogenetic relationship of macaques to humans has resulted in their widespread use as a preclinical model for bone marrow transplantation and stem cell gene therapy. To facilitate further use of this model, we undertook analysis of hematopoietic cells using multiparametric flow cytometric analysis. Rhesus CD34 + CD38 − cells displayed a number of characteristics of primitive hematopoietic cells, including low forward and orthogonal scatter and the lack of expression of lineage‐specific markers or human lymphocyte antigen‐DR. Four‐color flow cytometric analysis demonstrated that rhesus CD34 + CD38 − cells were heterogenous with respect to Thy‐1 expression and were CD59 dim . Quantitative limiting dilution long‐term culture‐initiating cell (LTC‐IC) analysis demonstrated that CD34 + CD38 − cells were approximately 150‐fold enriched for LTC‐IC as compared with unfractionated bone marrow, and occurred at a frequency similar to that previously reported in humans. Thus, as in humans, the CD34 + 38 − population of rhesus macaque bone marrow is enriched for primitive, multipotent hematopoietic progenitor cells.