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Immune responses following simian/human immunodeficiency virus (SHIV) challenge of rhesus macaques after human immunodeficiency virus (HIV)‐1 third variable domain (V3) loop‐based genetic immunization
Author(s) -
Le Borgne Sylvie,
Le Grand Roger,
Michel MarieLouise,
Vaslin Bruno,
Boson Bertrand,
Janvier Geneviève,
Aubertin AnneMarie,
Dormont Dominique,
Rivière Yves
Publication year - 2000
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.2000.290601.x
Subject(s) - virology , viremia , simian immunodeficiency virus , biology , immunology , immune system , immunization , virus , cd8 , cytotoxic t cell , antibody , genetics , in vitro
Following DNA immunization of rhesus macaques with a plasmid encoding the human immunodeficiency virus (HIV)‐1 third variable domain (V3) loop, presented by pseudo‐viral envelope particles of hepatitis B virus, specific immune responses were induced. The primates were then inoculated with a chimeric simian/human immunodeficiency virus (SHIV). All the animals were infected, but the V3‐specific immunization provided a relative attenuation of the acute phase of infection in the absence of neutralizing antibody. In all animals, SHIV‐specific cytotoxic T‐lymphocyte precursors (CTLp) were detected early in peripheral blood and lymph nodes. The viremia peak correlated significantly with the decrease in CD4+ T cells and with a transient increase in the percentage of natural killer cells. The infection induced an oligoclonalization of the CD8+ T‐cell variable β chain repertoire in the blood. Surprisingly, HIV envelope‐specific CTLp generated by genetic immunization may be governed by distinct circulation rules compared to SHIV‐specific CTLp induced by infection.