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Measurement of serum bone‐specific alkaline phosphatase activity in cynomolgus macaques
Author(s) -
Brommage Robert,
Allison Carolyn,
Stavisky Ronda,
Kaplan Jay
Publication year - 1999
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1999.tb00281.x
Subject(s) - alkaline phosphatase , immunoassay , endocrinology , medicine , bone remodeling , chemistry , microtiter plate , enzyme , biology , biochemistry , immunology , antibody , chromatography
Serum levels of bone‐specific alkaline phosphatase activity (B‐ALP) in cynomolgus monkeys were evaluated as an index of elevated bone turnover following ovariectomy. The enzyme immunoassay 96‐well microtiter plate B‐ALP assay, developed by Metra Biosystems (Mountain View, CA) for human use, was employed and compared with a standard automated assay measuring total serum levels of alkaline phosphatase activity (T‐ALP). The B‐ALP assay was first validated for use in these monkeys. Ovariectomy led to increased bone turnover as indicated by approximately 2‐fold higher activity in both assays and this elevation was inhibited by daily estradiol administration. Although both assays provided generally similar results, several monkeys were observed to have greatly elevated values of T‐ALP but not B‐ALP. This discrepancy is believed to result from high levels of the liver isoform of alkaline phosphatase in monkeys with hepatic dysfunction, which are not detected by the B‐ALP assay.