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Immunization against SIVmne in macaques using multigenic DNA vaccines
Author(s) -
Mossman Sally P.,
Pierce Christopher C.,
Robertson Michael N.,
Watson Andrew J.,
Montefiori David C.,
Rabin Michael,
Kuller LaRene,
Thompson Jannelle,
Lynch John B.,
Morton William R.,
Benveniste Raoul E.,
Munn Robert,
Hu ShiuLok,
Greenberg Philip,
Haigwood Nancy L.
Publication year - 1999
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1999.tb00271.x
Subject(s) - dna vaccination , immunogenicity , virology , biology , peripheral blood mononuclear cell , virus , macaque , recombinant dna , immunization , priming (agriculture) , antibody , immunology , gene , in vitro , genetics , paleontology , germination , botany
All structural and regulatory genes of SIVmne were cloned into mammalian expression vectors to optimize expression in vitro and immunogenicity in mice. Macaca fascicularis were immunized four times with plasmid DNA (n = 4), or two DNA priming inoculations followed by two boosts of recombinant gp160 plus Gag‐Pol particles (n = 4). Following intrarectal challenge with SIVmne, all macaques became infected. Three monkeys immunized with DNA alone maintained low plasma virus loads by 1 year post‐challenge; the fourth exhibited high virus loads and significant CD4 + cell decline. Two of the DNA plus boost and three control macaques had high virus loads and associated CD4 + cell decline. Both vaccine protocols elicited antibodies and comparable helper T‐cell proliferative responses to gp160. Cytokine mRNA levels in activated peripheral blood mononuclear cells (PBMC) taken at time of challenge suggested a dominant T helper (Th) 1 state in three DNA‐immunized and one protein‐boosted macaque, which correlated with low virus loads and high CD4 + cell counts post‐challenge.