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Evaluation of anti‐human antibodies for immunohistochemistry on archival nonhuman primate tissues
Author(s) -
Wykrzykowska Joanna J.,
Pauley Douglas R.,
Lackner Andrew A.,
Simon Meredith A.
Publication year - 1996
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1996.tb00197.x
Subject(s) - immunohistochemistry , antibody , nonhuman primate , antigen , primate , biology , pathology , specific antibody , immunology , medicine , evolutionary biology , ecology
A panel of commercially available antibodies which recognize specific antigens on human tissues was developed for use in immunohistochemistry on tissues from eight species of nonhuman primates. Antibodies were selected for potential usefulness in diagnostic pathology, and for effectiveness in formalin‐fixed, paraffin‐embedded tissues. Tissues from four species of macaques and four New World monkeys were evaluated. Using these antibodies we were able to identify 17/21 antigens examined in all eight species, and 21/21 antigens in the four species of macaques. Detailed immunohistochemistry protocols are presented, along with a systematic approach to developing a protocol for a new antibody.