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Characterization of the natural immune response of rhesus monkey CD4 +ve T cells to the bacterial antigen streptolysin O (SLO)
Author(s) -
Kraakman Elizabeth M.,
Bontrop Ronald E.,
Groenesteln Reno,
Jonker Margreet,
Haaijman Joost J.,
Hart Bert A. ‘t
Publication year - 1995
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1995.tb00183.x
Subject(s) - streptolysin , immune system , antigen , immunology , biology , microbiology and biotechnology , virology , bacteria , bacterial protein , genetics
Rhesus monkeys show a high proliferative T cell response to the bacterial exotoxin SLO without prior immunization. The present study was undertaken to characterize this naturally present SLO‐responsiveness with particular emphasis on CD4 +ve reactive T cells. It is demonstrated that the frequency of SLO‐reactive cells in the circulation ranges between 1 in 75 and 1 in 610 CD4 +ve T cells as determined with limiting dilution analysis. It is also shown that induction of a good proliferative response requires Mhc‐DR matching between T cell and the antigen presenting cells (APC). Stable and DR ‐restricted SLO‐specific CD4 +ve T cell lines were generated from CD8 depleted peripheral blood mononuclear cells (PBMC). The SLO‐reactive CD4 +ve cell lines are tentatively characterized as Thl‐like based on the predominant production of interferon‐gamma (IFN‐γ) over IL‐4, although this seems contradicted by the IL‐4 dependent growth of the lines.