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Assessing genetic‐based therapies for AIDS using the simian immunodeficiency virus
Author(s) -
Endres Carla L.,
Bergquam Eric,
Axthelm Michael K.,
Wong Scott W.
Publication year - 1995
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1995.tb00159.x
Subject(s) - simian immunodeficiency virus , virology , clone (java method) , transfection , biology , peripheral blood mononuclear cell , virus , plasmid , rhesus macaque , viral replication , macaque , simian , mutant , cell culture , gene , genetics , in vitro , paleontology
A plasmid encoding the full‐length infectious molecular proviral clone of SIV mac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4 + cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co‐transfection studies were initiated with the infectious proviral clone and a well‐characterized trans‐dominant negative HIV‐1 rev mutant.