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Production and characterization of murine monoclonal antibodies specific for baboon IgG heavy and light chain epitopes
Author(s) -
Shearer M.H.,
Jenson H.B.,
Carey K.D.,
Chanh T.C.,
Kennedy R.C.
Publication year - 1994
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1994.tb00124.x
Subject(s) - baboon , epitope , monoclonal antibody , immunoglobulin light chain , subclass , antibody , microbiology and biotechnology , biology , immune system , virology , western blot , chemistry , immunology , biochemistry , gene , endocrinology
We have generated a panel of murine monoclonal antibodies (MAbs) that recognize baboon IgG epitopes. The reactivity of the MAbs with IgG from other primate species was also examined. Specificity for IgG heavy (H) or light (L) chain epitopes was determined by Western blot analysis. The H chain‐specific MAbs were analyzed for IgG subclass specificity and the L chain‐specific MAbs for reactivity with baboon IgM and polymeric sIgA. Finally, an ELISA was developed to demonstrate the utility of the MAbs in analysis of humoral immune responses in baboons.