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An Hsp60 related protein is associated with purified HIV and SIV
Author(s) -
Bartz Steven R.,
Pauza C. David,
Ivanyi Juraj,
Jindal Satish,
Welch William J.,
Malkovsky Miroslav
Publication year - 1994
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1994.tb00116.x
Subject(s) - polyethylene glycol , peg ratio , hsp60 , heat shock protein , simian immunodeficiency virus , sepharose , size exclusion chromatography , hsp70 , virology , human immunodeficiency virus (hiv) , centrifugation , precipitation , blot , chemistry , affinity chromatography , biology , chromatography , biochemistry , enzyme , meteorology , economics , gene , physics , finance
The possible physical association of heat shock proteins (Hsp's) with immunodeficiency viruses (HIV and SIV) has been examined. The virions were purified by a) polyethylene glycol (PEG) precipitation and Sepharose 4B filtration, b) PEG precipitation and centrifugation over a Renografin gradient, or c) PEG precipitation and Matrex Cellufine Sulfate affinity chromatography. Western blotting revealed an Hsp60 related protein associated with HIV and SIV. Other Hsp's (such as Hsp70) were not detected, suggesting a specific interaction between Hsp60 and viral factors.