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Antiviral activity of primate γδ T lymphocytes isolated by magnetic cell sorting
Author(s) -
Wallace Marianne,
Gan YunnHwen,
Pauza C. David,
Malkovsky Miroslav
Publication year - 1994
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1994.tb00113.x
Subject(s) - peripheral blood mononuclear cell , monoclonal antibody , virology , priming (agriculture) , biology , antigen , immunology , in vitro , antibody , cell sorting , microbiology and biotechnology , flow cytometry , biochemistry , botany , germination
The exposure of human or rhesus monkey peripheral blood mononuclear cells (PBMCs) to interleukin 2 (IL‐2) in vitro resulted in a selective outgrowth of γδ lymphocytes. Using positive selection by monoclonal antibodies and magnetic beads, γδ T lymphocytes were isolated from these cultures. Without priming by viral antigens, the purified γδ T lymphocytes lyse immunodeficiency virus‐infected cells substantially better than the uninfected counterparts.