Premium
Quantitative study of chimpanzee and gorilla counterparts of the human D antigen
Author(s) -
Roubinet F.,
Blancher A.,
Socha W.W.,
Ruffié J.
Publication year - 1993
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1993.tb00633.x
Subject(s) - gorilla , epitope , monoclonal antibody , antigen , biology , immunofluorescence , antibody , papain , human blood , microbiology and biotechnology , enzyme , immunology , biochemistry , physiology , paleontology
The reactivities of three human anti‐D monoclonal antibodies (mAbs) with human, chimpanzee, and gorilla red blood cells (RBCs) were compared by quantitative radioimmunology and indirect immunofluorescence methods. The number of antigenic sites varies widely in gorillas (from 48,000‐283,000), while in chimpanzees this number is very close to that observed in human R 1 R 2 RBCs. The affinity of the anti‐D antibodies was slightly lower with ape RBCs than with D‐positive human RBCs. In chimpanzee, the D‐like epitopes recognition is enhanced by papain while the gorilla D‐like epitopes are destroyed by enzyme treatment.