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Detection of simian immunodeficiency virus DNA in macrophages from infected rhesus macaques
Author(s) -
Unger Ronald E.,
Marthas Marta L.,
Lackner Andrew A.,
PrattLowe Elissa,
Lohman Barbara L.,
Van Rompay Koen,
Luciw Paul A.
Publication year - 1992
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1992.tb00571.x
Subject(s) - simian immunodeficiency virus , virology , biology , virus , simian , macrophage , rhesus macaque , monocyte , immunology , genetics , in vitro
We have examined the frequency of infection of monocyte‐derived and alveolar macrophages isolated from rhesus macaques inoculated with simian immunodeficiency virus (SIV mac ) utilizing a semiquantitative PCR methodology. Animals were inoculated with either pathogenic (SIV mac239 ) or nonpathogenic (SIV mac1A11 ) molecularly cloned viruses of SIV mac , or with uncloned pathogenic SIV macBIOL . The frequency of SIV DNA in macrophages was highest early after infection and at terminal stages of disease, whereas during the asymptomatic period, SIV DNA was present at very low levels in macrophages.