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Isolation of functional polymorphonuclear leukocytes from rhesus monkeys using discontinuous Ficoll‐Hypaque density gradients
Author(s) -
Taniuchi Shoichiro
Publication year - 1991
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1991.tb00533.x
Subject(s) - ficoll , chemotaxis , granulocyte , immunology , isolation (microbiology) , chemistry , biology , microbiology and biotechnology , biochemistry , in vitro , peripheral blood mononuclear cell , receptor
A simple, rapid method for separating polymorphonuclear leukocytes (PMNs) from adult rhesus monkey blood, based on the use of a discontinuous gradient of Ficoll‐Hypaque (densities, 1.100, 1.077) has been developed. Using this method, 71.1% of the PMNs were recovered in a layer with a purity of 91% PMNs with very few contaminating erythrocytes. The added advantage of the method described here is that a higher chemotactic activity was retained.