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Recovery of the simian immunodeficiency virus (SIV) and depression of colony formation in in vitro infected progenitor cell‐enriched rhesus bone marrow (BM)
Author(s) -
Beltz Lisa,
Narayan Opendra,
Adams Robert J.,
Noga Stephen J.,
Donnenberg Albert D.
Publication year - 1991
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1991.tb00509.x
Subject(s) - simian immunodeficiency virus , clonogenic assay , progenitor cell , biology , virology , in vitro , bone marrow , virus , haematopoiesis , immunology , lymphoblast , cell culture , stem cell , microbiology and biotechnology , genetics , biochemistry
Rhesus progenitor‐enriched BM was exposed overnight to SIV and cultured in a limiting dilution assay where the potential for progenitor interaction with lymphocytes or macrophages was low. Virus was consistently isolated late in culture, detection being aided by coculture with CEM 174 lymphoblasts. Although infected cells had reduced clonogenic activity, colonies were indistinguishable from those derived from uninfected BM with respect to proliferative potential, morphology, and longevity in culture. Primate immunodeficiency viruses, therefore, may infect immature BM populations, directly affecting hematopoietic activity.