Premium
Quantitation of serum immunoglobulins G, M, and A in the rhesus monkey (M. mulatto) using human monospecific antisera in the enzyme‐linked immunosorbent assay: Developmental aspects
Author(s) -
Tryphonas Helen,
Karpinski Kazimierz,
O'Grady Lois,
Hayward Stephen
Publication year - 1991
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1991.tb00493.x
Subject(s) - antiserum , antibody , biology , immunology
Using human monospecific antisera, several parameters have been optimized for the micro‐ELISA “sandwich” technique used in the quantitative measurement of total serum IgG, IgM and IgA levels in rhesus monkeys. Representation of the optical density as a four‐parameter logistic function provided excellent fits of the data over a wide choice of dilutions of human antisera used for coating the ELISA plates and for the peroxidase‐conjugated antisera used in the system. The mirco‐ELISA “sandwich” technique was shown to be specific, reliable, sensitive, and economical for use in the routine measurement of total serum IgG, IgM and IgA levels in the rhesus monkey.