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Nef Genes of SIV
Author(s) -
Kestler H.W.,
Mori K.,
Silva D.P.,
Kodama T.,
King N.W.,
Daniel M.D.,
Desrosiers R.C.
Publication year - 1990
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1990.tb00447.x
Subject(s) - biology , reversion , virus , virology , gene , stop codon , mutant , coding region , transfection , in vivo , genetics , rhesus macaque , microbiology and biotechnology , phenotype
Molecular clones of SIVmac were constructed that differed only in sequences within the nef gene. DEAE‐transfection of viral DNA containing an open form of /ic/yiclded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef. Rhesus monkeys that received each kind of molecularly cloned virus became infected. Our results additionally suggest that mutant forms of virus are selected in vitro while open, functional forms are selected in vivo. In animals infected with virus containing a stop codon within nef, reversion of the stop codon to a coding codon was demonstrated in live of five clones analyzed. These results indicate that nef is playing some role crucial to the virus life cycle in vivo.