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Molecular Interactions of Cytomegalovirus and the Human and Simian Immunodeficiency Viruses
Author(s) -
Barry Peter A.,
PrattLowe Elissa,
Linger Ronald E.,
Marthas Marta,
Alcendor Donald J.,
Luciw Paul A.
Publication year - 1990
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1990.tb00439.x
Subject(s) - simian immunodeficiency virus , chloramphenicol acetyltransferase , virology , transactivation , biology , coinfection , cytomegalovirus , human cytomegalovirus , reporter gene , long terminal repeat , gene , gene expression , human immunodeficiency virus (hiv) , virus , viral disease , genetics , herpesviridae
In HuT 78 cells chronically infected with SIV, super‐infection with rhesus cytomegalovirus (rhCMV) stimulated an increase in SIV replication. Utilizing transient expression assays with the SIV long terminal repeat (LTR) driving expression of the chloramphenicol acetyltransferase (CAT) reporter gene, the increase in SIV replication, by coinfection with CMV, was due to transactivation of the SIV LTR by the immediate early gene products (IE) of rhesus CMV. Similarly, IE of human CMV stimulated expression from both the SIV and HIV LTRs.