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Sequence Variability of Simian Immunodeficiency Virus in a Persistently Infected Rhesus Monkey
Author(s) -
Burns Dawn P.W.,
Desrosiers Ronald C.
Publication year - 1990
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1990.tb00438.x
Subject(s) - simian immunodeficiency virus , virology , simian , sequence (biology) , biology , human immunodeficiency virus (hiv) , immunodeficiency , virus , immunology , genetics , immune system
A juvenile rhesus monkey that was inoculated intravenously with molecularly cloned SIVmac239 became persistently infected. A modified polymerase chain reaction (PCR) procedure was used to specifically amplify full‐length envelope (env) gene sequences from DNA extracted from peripheral blood mononuclear cells (PBMC), lymph node tissue, and cells infected with recovered virus at 69 and 93 weeks post‐infection. Extensive sequence variability accumulated in vivo in spite of infection with molecularly cloned virus. In the central portion of env, sequence variability was largely confined to three discrete regions.