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Mutational Analysis of Simian Immunodeficiency Virus From African Green Monkeys and Human Immunodeficiency Virus Type 2
Author(s) -
Shibata Riri,
Adachi Akio,
Sakai Hiroyuki,
Ishimoto Akinori,
Miura Tomoyuki,
Hayami Masanori
Publication year - 1990
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1990.tb00429.x
Subject(s) - virology , biology , infectivity , transactivation , simian immunodeficiency virus , virus , mutant , wild type , transfection , serial passage , gene , microbiology and biotechnology , gene expression , genetics
We constructed ten mutants of simian immunodeficiency virus isolated from African green monkey (SIV AGM ), and nine mutants of human immunodeficiency virus type 2 (HIV‐2) in vitro. Their infectivity, cytopathogenicity, transactivation potential, virus RNA, and protein synthesis were examined by transfection and infection experiments. Mutations in three structural (gag, pol, env) and two regulator (tat, rev) genes abolished the infectivity of both viruses, but vpx, vpr (HIV‐2), and nefv/crc dispensable and mutant viruses were indistinguishable phenotypically from wild type virus. A vt/mutant of HIV‐2 showed poor infectivity in cell‐free condition, whereas SIV AGM mutants grew equally well with wild type virus. In transient transfection assays, rev mutants derived from both viruses produced mainly small mRNA species and no detectable virus proteins and particles. Transactivation potential of tat mutants originated from both viruses was about three‐ to ten‐fold less than that of respective wild type DNAs, generating small amounts of virus.