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The Simian Immunodeficiency Virus (SIV) rev Gene Regulates Env Expression
Author(s) -
Cheng SheauMei,
Blume Marlene,
Lee ShawGuang,
Hung Paul P.,
Hirsch Vanessa M.,
Johnson Philip R.
Publication year - 1990
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1990.tb00425.x
Subject(s) - simian immunodeficiency virus , virology , gene , biology , syncytium , gene expression , gene product , virus , monoclonal antibody , rna , messenger rna , microbiology and biotechnology , simian , lentivirus , antibody , genetics , viral disease
The rev gene product is a trans‐acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev + env + construct, and pBD21 is a rev − env + construct. After transfecting the constructs into COS‐1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full‐length env mRNA and was required for expression of SIV gp160.