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Human Immunodeficiency Virus (HIV) From Experimentally Infected Chimpanzees: Isolation and Characterization
Author(s) -
Castro B.A.,
Walker C.M.,
Tateno M.,
ChengMayer C.,
Heberling R.,
Eichberg J.W.,
Levy J.A.
Publication year - 1989
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1989.tb00236.x
Subject(s) - virology , biology , virus , clone (java method) , viral replication , restriction enzyme , human immunodeficiency virus (hiv) , isolation (microbiology) , host (biology) , microbiology and biotechnology , dna , genetics
Human immunodeficiency virus‐1 (HIV‐1) isolates obtained from chimpanzees that had undergone various immunosuppressive treatments were characterized by growth on various primary cells and cell lines as well as by restriction endonuclease analysis. Viruses recovered from animals inoculated with uncloned HIV showed genetic variation from the original inoculum, whereas viruses isolated from an animal infected with a molecular clone of HIV did not. In some cases, virus recovery was possible only after enrichment for CD4 + cells by panning, inoculation with a chimpanzee cytomegalovirus, or a combination of these procedures. These findings indicate a role for viral and host cofactors in the control of virus replication and suggest explanations for the absence of clinical manifestations in HIV‐infected chimpanzees.