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Establishment of Primate Lymphoblastic Cell Lines by Coculture With a Simian T‐Cell Leukemia Virus‐1 Positive, Hypoxanthine‐Guanine‐Phosphoribosyltransferase Negative Japanese Macaque Cell Line
Author(s) -
Murayama Yuichi,
Ishida Takafumi,
Minezawa Mitsuru,
Noguchi Atsuo,
Takenaka Osamu
Publication year - 1988
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1988.tb00357.x
Subject(s) - hypoxanthine guanine phosphoribosyltransferase , simian , virology , cell culture , macaque , primate , biology , rhesus macaque , phosphoribosyltransferase , hypoxanthine , virus , microbiology and biotechnology , cancer research , genetics , biochemistry , neuroscience , enzyme , gene , mutant
A cell line (JAMH17 + ) resistant to 8‐azaguanine was established from a human T‐cell leukemia virus type 1 related virus (simian T‐cell leukemia virus‐1) positive Japanese macaque cell line. Lymphoblastic cell lines were established from the peripheral blood mononuclear cells of humans, hominoids, and several species of macaques by coculture with JAMH17 + in hypoxanthine‐aminopterin‐thymidine medium. HTLV‐1 specific antigen was detected in some of the established cell lines. Phenotypic analysis showed that several cell lines of crab‐eating macaques expressed Leu11a antigen, which is a marker of human natural killer cells.