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Determination of Ovulation and Pregnancy in the Marmoset ( Callithrix jacchus ) by Monitoring of Urinary Hydroxypregnanolone Excretion
Author(s) -
Heger W.,
Neubert D.
Publication year - 1987
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1987.tb00325.x
Subject(s) - marmoset , callithrix , excretion , ovulation , urinary system , luteal phase , luteinizing hormone , medicine , endocrinology , urine , metabolite , pregnancy , biology , hormone , chemistry , physiology , paleontology , genetics
In Callithrix jacchus the urinary excretion of hydroxypregnanolone (5α‐pregnane‐3α,7α‐diol‐20‐one) shows a luteal rise during the ovulatory cycle. This progesterone metabolite can therefore be used as an indication of ovulation, implantation, and subsequently for the persistence of the pregnancy. The excretion can be monitored by high performance thin‐layer chromatography (HPTLC) and postchromatographic derivatization after enzymatic hydrolysis of the conjugate. Profiles of urinary hydroxypregnanolone levels are presented and correlated with luteinizing hormone excretion. The method is noninvasive and therefore suited for long‐term studies in these monkeys.