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Characterization of Various Molecular Species of Follicle‐Stimulating Hormone in Baboon Pituitary Preparations
Author(s) -
Khan S.A.,
Katzija G.,
Fröysa B.,
Diczfalusy E.
Publication year - 1984
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1984.tb00153.x
Subject(s) - baboon , anterior pituitary , follicle stimulating hormone , biology , in vitro , pituitary gland , hormone , endocrinology , medicine , chemistry , biochemistry , chromatography , luteinizing hormone
The properties of baboon ( Papio hamadryas ) follicle‐stimulating hormone (bFSH) have been studied after isoelectrofocusing (IEF) of individual pituitary extracts from five female and three male baboons and of a partially purified bFSH preparation (CM‐1). The in vitro bioactivities of the female and male pituitary extracts and of CM‐1 were 6.0 (range 4.1–9.6), 10.8 (6.9–18.2), and 34.1 (30.3–38.3) mg equivalent of LER 1909‐2 reference standard per mg protein, respectively. The corresponding ratios of bioactivity to immunoreactivity (B/I) were 1.72 (range 1.31–2.06), 1.82 (1.53–2.25), and 1.54 (1.51–1.58), respectively. There was a significant increase (p < 0.05) in all B/I ratios after IEF, due to a diminished immunoreactivity without any loss in bioactivity. Several molecular forms of bFSH were observed with pI values ranging from 4.5 to 7.5, with maximum activity between pH 4.5 to 6.0. The IEF profiles of female and male pituitaries and of the purified bFSH preparation were similar. However, the B/I ratios of the different molecular species of bFSH increased with increasing pI values in every case. It is concluded that there is a significant heterogeneity of bFSH in crude as well as in purified pituitary extracts, but neither the sex of the animals nor the process of purification influenced the quantity and distribution of various molecular species.