Premium
Biological and Immunological Characterization of Purified Follicle‐Stimulating Hormone Preparations From Monkey Pituitaries
Author(s) -
Khan Shafiq A.,
Fröysa Berit,
Diczfalusy Egon
Publication year - 1984
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/j.1600-0684.1984.tb00147.x
Subject(s) - radioimmunoassay , bioassay , heterologous , immunoassay , follicle stimulating hormone , fractionation , hormone , chemistry , biology , chromatography , endocrinology , medicine , luteinizing hormone , antibody , biochemistry , immunology , genetics , gene
The suitability of a purified rhesus (rhFSH) and cynomolgus (cynFSH) follicle‐stimulating hormone (FSH) preparation to replace the expired LER 1909‐2 reference standard in the immunoassay of monkey FSH was assessed using an in vitro bioassay and two heterologous radioimmunoassay (RIA) procedures in combination with fractionation by high‐resolution isoelectrofocusing. The results presented indicate that rhFSH is unsuitable for the replacement of LER 1909‐2 as a standard, though cynFSH might be suitable after the removal of its LH contamination.