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The mouse major histocompatibility complex: some assembly required
Author(s) -
Amadou Claire,
Kumánovics Attila,
Jones Elsy P.,
LambrachtWashington Doris,
Yoshino Masayasu,
Lindahl Kirsten Fischer
Publication year - 1999
Publication title -
immunological reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.839
H-Index - 223
eISSN - 1600-065X
pISSN - 0105-2896
DOI - 10.1111/j.1600-065x.1999.tb01394.x
Subject(s) - contig , biology , genetics , major histocompatibility complex , synteny , bacterial artificial chromosome , yeast artificial chromosome , gene , mhc class i , chromosome , gene mapping , genome
Summary: We have assembled a contig of 81 yeast artificial chromosome clones that spans 8 Mb and contains the entire major histocompatibility complex (Mhc) from mouse strain C57BL/6 (H2 b ), and we are in the process of assembling an Mhc contig of bacterial artificial chromosome (BAC) clones from strain 129 (H2 bc ), which differs from C57BL/6 in the H2‐Q and H2‐T regions. The current BAC contig extends from Tapasin to D17Leh89 with gaps in the class II, H2‐Q. and distal H2‐M regions. Only four BAC clones were required to link the class I genes of the H2‐Q and H2‐T regions, and no new class I gene was found in the previous gap. The proximal 1 Mb of the H2‐M region has been analyzed in detail and is ready for sequencing; it includes 21 class I genes or fragments, at least 14 olfactory receptor‐like genes, and a number of non‐class I genes that clearly establish a conserved synteny with the class I regions of the human and rat Mhc.