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Induction of Human Antibody Responses In Vitro with Emphasis on Allogeneic Helper Factors
Author(s) -
Chiorazzi Nicholas,
Fu Shu Man,
Kunkel Henry G.
Publication year - 1979
Publication title -
immunological reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.839
H-Index - 223
eISSN - 1600-065X
pISSN - 0105-2896
DOI - 10.1111/j.1600-065x.1979.tb00279.x
Subject(s) - medicine , leukemia , gerontology , library science , immunology , classics , history , computer science
Systems have been developed for the reproducible production of antigen-specific plaque forming cells for both tonsillar and peripheral blood B cells. Allogeneic helper activity was an essential supplement and monocyte removal was important, especially in the peripheral blood situation. Highly active allogeneic helper factors could be obtained from undirectional MLC supernatants which aided the proeuction of antigen-driven plaques. These factors also caused a polyclonal activation of B lymphocytes. A number of clinical applications of the above system were described. A defect in the T cells of patients with chronic lymphocytic leukemia with respect to the generation of helper activities was delineated.