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Evaluation of ovum storage techniques for reproductive studies of fishes
Author(s) -
Heins D. C.,
Baker J. A.
Publication year - 1999
Publication title -
ecology of freshwater fish
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 55
eISSN - 1600-0633
pISSN - 0906-6691
DOI - 10.1111/j.1600-0633.1999.tb00055.x
Subject(s) - isopropyl alcohol , etheostoma , biology , alcohol , reproduction , zoology , andrology , ecology , fishery , chemistry , fish <actinopterygii> , biochemistry , medicine , organic chemistry
– We compared dry masses of mature oocytes and ripe eggs from Etheostoma lynceum and Etheostoma caeruleum that were stored in varying concentrations of either formalin or isopropyl alcohol. In comparison to 10% formalin, alcohol significantly reduced the weights of both mature oocytes and ripe eggs, with 25% isopropyl alcohol resulting in much smaller reductions than 50% isopropanol. Three percent formalin resulted in smaller decreases in the masses of oocytes and eggs than the alcohol treatments, and not all comparisons with 10% formalin were significantly different. We recommend fixing specimens and storing them and gametic cells removed from them in 10% formalin. In cases where specimens or eggs in alcohol must be used in addition to material in formalin, correction factors (to formalin standard) should be developed, but the correction should not be made uncritically due to the varying effects alcohol may have among samples.