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P olypodium leucotomos decreases UV ‐induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice
Author(s) -
RodríguezYanes Esperanza,
Juarranz Ángeles,
Cuevas Jesús,
Gonzalez Salvador,
Mallol Jordi
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2012.01544.x
Subject(s) - hairless , antioxidant , western blot , pharmacology , skin aging , endogeny , cell growth , chemistry , systemic administration , biochemistry , biology , medicine , dermatology , gene , microbiology and biotechnology , in vivo
A single dose of ultraviolet radiation ( UVR ) induces significant changes in blood and skin of hairless mice. Oral administration of a hydrophilic extract of the fern P olypodium leucotomos ( PL , 300 mg/kg during 5 days before UVR and for two additional days after irradiation) modulates some of the effects of UVR . Most significantly, PL administration reduced the number of proliferating cells by 13%, increased the number of p53 + cells by 63%, enhanced the antioxidant plasma capacity ( ORAC ) by 30% and reinforced the network of dermal elastic fibres. Western blot analysis of skin antioxidant‐related enzymes failed to demonstrate significant changes caused by PL . Thus, the beneficial effect of PL likely owes to its antioxidant and anti‐ ROS properties rather than its modulation of the expression of endogenous antioxidant systems. These data provide mechanistic clues for its efficacy as a systemic photoprotective agent with antioxidant and anti‐photo‐ageing properties.