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Anti‐ageing effects of alpha‐naphthoflavone on normal and UVB ‐irradiated human skin fibroblasts
Author(s) -
Liao PoLin,
Li ChingHao,
Chang ChengYi,
Lu SiangRong,
Lin ChengHui,
Tse LingShan,
Cheng YuWen
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2012.01517.x
Subject(s) - ageing , mapk/erk pathway , human skin , chemistry , skin aging , downregulation and upregulation , microbiology and biotechnology , fibroblast , signal transduction , cancer research , biochemistry , biology , medicine , in vitro , genetics , dermatology , gene
Ageing is a complex and multifactorial process resulting in several functional and aesthetic changes to the skin. We found that α‐Naphthoflavone (α‐ NF ) concentration‐dependently induced pro‐collagen type I protein expression and inhibited MMP ‐1 protein expression, in both normal and UVB ‐irradiated cells. SB 431542 and SIS 3 – inhibitors of TGF ‐β and S mad3, respectively – significantly alleviate α‐ NF ‐caused response of MMP ‐1 and pro‐collagen. LY 294002 ( PI 3K inhibitor) can reverse α‐ NF ‐induced ERK , A kt, S mad‐3 activation, pro‐collagen synthesis and α‐ NF ‐suppressed AP ‐1 activation. PD ( ERK inhibitor) was not involved in pro‐collagen generation and MMP ‐1 inhibition. We concluded that α‐ NF promotes pro‐collagen production and inhibits MMP ‐1 expression via the activation of a PI 3 K / A kt/ S mad‐3 pathway in normal and UVB ‐irradiated human skin fibroblasts, while TGF ‐β may play an important role in transducing this pathway. These results suggest that α‐ NF , a natural plant product, has the potential to become a novel anti‐ageing skin application.