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Immortalized sebocytes can spontaneously differentiate into a sebaceous‐like phenotype when cultured as a 3D epithelium
Author(s) -
Barrault Christine,
Dichamp Isabelle,
Garnier Julien,
Pedretti Nathalie,
Juchaux Franck,
Deguercy Alain,
Agius Gérard,
Bernard FrançoisXavier
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2012.01463.x
Subject(s) - cytokeratin , keratinocyte , sebaceous gland , microbiology and biotechnology , epithelium , phenotype , cell culture , biology , human skin , cellular differentiation , immortalised cell line , cell , chemistry , endocrinology , immunology , immunohistochemistry , genetics , gene
Sebocytes originate from the same lineage as keratinocytes, and both cell types may have similarities in terms of growth and differentiation. We were interested in studying the behaviour of human sebocytes when cultured in conditions validated for epidermal reconstruction. For this purpose, we established a HPV16‐E6/7‐immortalized human sebocyte cell line (SEBO662) growing in keratinocyte defined media. Postconfluent SEBO662 cells in monolayers express the early sebocyte marker, cytokeratin 7 (K7), do not express Epithelia Membrane Antigen (EMA) and do not exhibit strong lipogenic activity. However, when placed at the air–liquid interface, SEBO662 multilayers spontaneously differentiate into a sebaceous‐like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium. This work highlights the value of simple 3D models for exhibiting spontaneous differentiation and polarization.