Premium
Hair‐inducing ability of human dermal papilla cells cultured under Wnt/β‐catenin signalling activation
Author(s) -
Soma Tsutomu,
Fujiwara Shigeyoshi,
Shirakata Yuji,
Hashimoto Koji,
Kishimoto Jiro
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2012.01458.x
Subject(s) - dermal papillae , wnt signaling pathway , hair follicle , catenin , microbiology and biotechnology , hair cycle , biology , human skin , beta catenin , cell culture , cell , signal transduction , biochemistry , genetics
It is well known that dermal papilla cells (DPCs) play crucial roles in hair follicle induction. In this study, we examined whether Wnt/β‐catenin activation results in maintenance of the hair‐inducing ability of human DPCs. Expression of DPC marker genes was maintained under Wnt/β‐catenin signalling stimulation by GSK‐3β inhibition. Furthermore, human DPCs showed constant hair induction when transplanted with murine epidermal cell fraction. Alu‐positive human DPCs were essentially detected adjacent to the reconstructing epidermal structure positive for P‐cadherin immunoreactivity. The transplanted human DPCs were abundant in the surrounding dermal sheath portion of the fully regenerated hair follicles. These results support the importance of Wnt/β‐catenin signalling in hair follicle induction. This study may provide valuable information to establish a culture method of human DPCs for cell‐based therapy.