Premium
Cost‐effective HLA‐Cw06:02 typing in a Caucasian population
Author(s) -
Nikamo Pernilla,
Ståhle Mona
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01423.x
Subject(s) - typing , human leukocyte antigen , population , medicine , genetics , biology , immunology , environmental health , antigen
Considering the clinical and genetic heterogeneity of psoriasis which may translate into distinct disease pathology and treatment response, correct typing of the main candidate gene HLA‐C is critical but not trivial. To facilitate genotyping, we compared established techniques with our newly developed tool. Here, we propose that typing of four single nucleotide polymorphic markers within the HLA‐C region correctly determines HLA‐Cw*06:02 genotypes in psoriatic cases and healthy controls in a population of Caucasian origin. Typing of the SNPs presented herein proved to be precise, reliable, time and cost effective, and requiring low amount of DNA.