Premium
Non‐invasive evaluation of dermal elastosis by in vivo multiphoton tomography with autofluorescence lifetime measurements
Author(s) -
Koehler Martin J.,
Preller Anja,
Elsner Peter,
König Karsten,
Hipler Uta C.,
Kaatz Martin
Publication year - 2012
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01405.x
Subject(s) - autofluorescence , in vivo , fluorescence , fluorescence lifetime imaging microscopy , materials science , biomedical engineering , chemistry , optics , medicine , biology , physics , microbiology and biotechnology
The non‐invasive differentiation of dermal elastic fibres from solar elastosis in vivo is of great interest in dermatologic research, especially for efficacy testing of anti‐ageing products. To date, no studies on multiphoton excited fluorescence lifetime characteristics of human elastic fibres and solar elastosis are reported. The goal of the present work was the identification of differential criteria for elastic fibres and solar elastosis by the analysis of fluorescence decay curves acquired by time‐correlated single photon counting in vivo multiphoton tomography. For this purpose, fluorescence lifetime measurements (FLIM) were performed with 47 volunteers of different age groups at sun‐protected and sun‐exposed localizations. Bi‐exponential curve fitting was applied to the FLIM data, and characteristic differences between age groups and localizations were found in both relevant fit parameters describing the decay slope. The FLIM analyses have shown that dermal autofluorescence has different lifetimes depending on age and in part on localization.