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Analysis of the 3′UTR of the prostaglandin synthetase‐2 (PTGS‐2 / COX‐2) gene in non‐melanoma skin cancer after organ transplantation
Author(s) -
GomezLira Macarena,
Tessari Gianpaolo,
Mazzola Silvia,
Malerba Giovanni,
Rugiu Carlo,
Naldi Luigi,
Nacchia Francesco,
Valerio Francesca,
Anna Brunello,
Forni Alberto,
Boschiero Luigino,
Sandrini Silvio,
Faggian Giuseppe,
Girolomoni Giampiero,
Turco Alberto
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01381.x
Subject(s) - skin cancer , organ transplantation , transplantation , cancer research , medicine , gene , melanoma , prostaglandin , oncology , cancer , biology , genetics
To define the potential involvement of polymorphisms in the 3′untranslated region (3′UTR) of the prostaglandin synthetase‐2 (PTGS‐2) gene to non‐melanoma skin cancer (NMSC) predisposition after transplantation, we screened for genetic variant, relevant parts of this region. It contains binding sites for trans‐acting factors, an alternative polyadenylation site and putative target sequences for miRNAs. Variant +8473T>C did not appear to play a functional role in the regulation of gene expression in human keratinocyte‐transfected cells. In addition to the well‐known +8473T>C, we identified four polymorphisms: +8293G>C, +10259T>G, +10267G>A and +10335G>A. No allele frequency differences were observed between cases and controls neither for +8473T>C nor for any of the identified polymorphisms, suggesting that polymorphisms in the 3′UTR of the PTGS2 gene are rare and unlikely to represent risk factor for NMSC after transplantation.