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Effects of topical application of EGCG on testosterone‐induced hair loss in a mouse model
Author(s) -
Kim Yoon Young,
up No Sun,
Kim Min Ho,
Kim Hei Sung,
Kang Hoon,
Kim Hyung Ok,
Park Young Min
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01353.x
Subject(s) - testosterone (patch) , hair loss , hair growth , chemistry , pharmacology , dermatology , medicine , endocrinology , physiology
We investigated the effect of topical epigallocatechin‐3‐gallate (EGCG) on testosterone (T)‐induced hair loss in mice. Marked hair loss was observed at the T‐injected site, and topical EGCG significantly reduced the hair loss ( P < 0.05). TUNEL staining showed apoptosis of follicular epithelial cells in the T‐injected groups where topical EGCG was found to significantly diminish T‐induced apoptosis ( P < 0.05). Topical EGCG down‐regulated the T‐induced expression of androgen receptor but did not down‐regulate 17β‐hydroxysteroid dehydrogenase (HSD) and three β‐HSD expression. Analysis using liquid chromatography tandem mass spectrometry (LC‐MS/MS) on serum and tissue samples revealed no significant difference in T and dihydrotestosterone concentrations between the T‐injected and T + EGCG groups. Thus, we found that T injection in a mouse model induces hair loss by apoptosis of the hair follicles rather than through the androgen metabolic pathway and also saw that T‐induced apoptosis of hair follicles was reduced by topical EGCG.