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Sphere formation restores and confers hair‐inducing capacity in cultured mesenchymal cells
Author(s) -
Shimizu Ruka,
Okabe Keisuke,
Kubota Yoshiaki,
NakamuraIshizu Ayako,
Nakajima Hideo,
Kishi Kazuo
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01281.x
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , hair follicle , dermis , in vivo , wnt signaling pathway , in vitro , dermal papillae , regeneration (biology) , morphogenesis , chemistry , biology , anatomy , signal transduction , biochemistry , gene , genetics
Interactions between epithelial and dermal cells are essential for hair follicle morphogenesis and maintenance. In experimental trials of hair regeneration, isolated dermal cells have been shown to possess hair‐inducing capacity. However, dermal cells lose this potential immediately after cultivation. Sphere‐forming multipotent cells derived from the dermis possess hair‐inducing capacity. These previous findings raise the question of whether hair‐inducing capacity depends on the identity as dermal cells or the process of sphere formation. To address this issue, we compared the in vitro and in vivo characteristics of two‐dimensionally cultured or thereafter sphere formation–induced dermal and lung mesenchymal cells. We show that sphere‐forming mesenchymal cells exhibited higher expression of Wnt signalling genes. Sphere‐forming cells but not two‐dimensionally cultured cells possessed in vivo hair‐inducing capacity. These data suggest that various mesenchymal cells attain hair‐inducing capacity through the process of sphere formation.