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2D luminescence imaging of physiological wound oxygenation
Author(s) -
Schreml Stephan,
Meier Robert J.,
Wolfbeis Otto S.,
Maisch Tim,
Szeimies RolfMarkus,
Landthaler Michael,
Regensburger Johannes,
Santarelli Francesco,
Klimant Ingo,
Babilas Philipp
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2011.01263.x
Subject(s) - wound healing , stratum corneum , chemistry , in vivo , oxygen , oxygenation , biomedical engineering , surgery , medicine , pathology , anesthesia , biology , microbiology and biotechnology , organic chemistry
In cutaneous wound healing, the role of oxygen in vivo is poorly understood. We studied wound surface pO 2 during physiological wound healing in humans. Split‐thickness skin graft donor sites ( n = 12) served as standardized wound models. Wound surface pO 2 was measured at 1, 6 and 14 days after split‐skin harvesting using two‐dimensional luminescence lifetime imaging (2D‐LLI) of palladium(II)‐meso‐tetraphenyl‐tetrabenzoporphyrin (Pd‐TPTBP) in polystyrene‐co‐acrylonitrile (PSAN) particles on transparent foils. In another experiment, we removed the stratum corneum (SC) on the volar forearm ( n = 10) by tape strippings to study the impact of the SC on the epidermal oxygen barrier. Split‐skin donor site pO 2 significantly decreased during the time course of physiological healing. Regional differences in pO 2 within donor site wounds were visualized for the first time in literature. No difference was found in pO 2 before and after SC removal, showing that the SC is not a major constituent of the epidermal oxygen barrier.