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In vivo response of GsdmA3Dfl/+ mice to topically applied anti‐psoriatic agents: effects on epidermal thickness, as determined by optical coherence tomography and H&E staining
Author(s) -
Zulfakar Mohd Hanif,
Alex Aneesh,
Povazay Boris,
Drexler Wolfgang,
Thomas Christopher P.,
Porter Rebecca M.,
Heard Charles M.
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01233.x
Subject(s) - in vivo , psoriasis , epidermis (zoology) , betamethasone dipropionate , keratinocyte , optical coherence tomography , betamethasone , chemistry , pathology , medicine , dermatology , endocrinology , biology , in vitro , ophthalmology , anatomy , biochemistry , microbiology and biotechnology
This study evaluated in vivo the potential of optical coherence tomography (OCT) to determine changes in thickness of the epidermis in response to the topically applied anti‐psoriatics betamethasone dipropionate (BD), salicylic acid (SA) and also fish oil (FO). GsdmA3Dfl/+ mice have an inflammatory hair loss phenotype that includes hyperproliferation and epidermal thickening, hence a potential psoriasis model. Changes in epidermal thickness were evaluated over a period of 10 days, with the mice treated with combined BD + SA, FO + SA and BD + FO + SA. The data were validated with conventional measurement using H&E staining coupled with microscopy. Initial baseline measurement revealed an average epidermal thickness of 26.92 ± 1.17 μm. After 10 days of treatment with BD, the average epidermal thickness was reduced by 38.8% ( P = 0.0001), and inversely, treatment with FO resulted in an unexpected 105% increase ( P = 0.0001) in epidermal thickness. Combined BD + FO treatment did not cause any significant change ( P = 0.3755) and may further indicate opposing effects on keratinocyte proliferation. The data obtained using OCT were statistically the same as those obtained by H&E/microscopy ( P = 0.4325), supporting a greater role for OCT in dermatological studies, while also allowing a reduction in the number of animals used in such studies as sacrifice at individual timepoints is not necessary.