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Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy
Author(s) -
Rankl Christian,
Zhu Rong,
Luengo Gustavo S.,
Donovan Mark,
Baghdadli Nawel,
Hinterdorfer Peter
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01179.x
Subject(s) - stratum corneum , atomic force microscopy , nanoscopic scale , linker , microscopy , surface (topology) , biophysics , chemistry , nanotechnology , materials science , optics , physics , computer science , biology , geometry , mathematics , operating system , genetics
Corneodesmosin, a protein known to be present in the stratum corneum (SC), plays an important role in its physical integrity. Here, a specific antibody to corneodesmosin was tethered via a flexible linker to an atomic force microscopy tip, and the interaction forces between this tip and the surface of the SC were successfully measured. Using the recently developed technique of simultaneous topography and recognition imaging, we were able to map the distribution of corneodesmosin on the surface of the SC at the nanoscale.