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Non‐thermal atmospheric‐pressure plasma can influence cell adhesion molecules on HaCaT‐keratinocytes
Author(s) -
Haertel Beate,
Wende Kristian,
von Woedtke Thomas,
Weltmann Klaus Dieter,
Lindequist Ulrike
Publication year - 2011
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01159.x
Subject(s) - hacat , cell adhesion , cell adhesion molecule , atmospheric pressure plasma , integrin , chemistry , adhesion , cell , microbiology and biotechnology , nonthermal plasma , flow cytometry , extracellular matrix , immunology , medicine , biology , biochemistry , in vitro , plasma , physics , organic chemistry , quantum mechanics
Non‐thermal atmospheric‐pressure plasmas provide new hope for improvement in chronic wound management because of their potency in killing microorganisms. However, the effectiveness of the procedure has to be verified and negative effects on healthy tissues have to be excluded. In wound healing adhesion molecules play a crucial role for cell migration and proliferation. We investigated whether an atmospheric‐pressure plasma jet (kINPen09) influences the expression of adhesion molecules responsible for cell‐cell and cell‐matrix interactions after treatment of HaCaT‐keratinocytes for 10 and 30 s. Twenty‐four hours after plasma treatment expression of α 2 ‐ and β 1 ‐integrin, E‐cadherin and the epidermal growth factor receptor (EGFR) was determined by flow cytometry. Plasma‐treated HaCaT‐cells were characterized by normal α 2 ‐integrin and increased β 1 ‐integrin expression. E‐cadherin and EGFR expression was reduced after the 30‐s treatment. We did not observe any effects following the 10‐s plasma treatment. In conclusion, short‐term plasma treatment can be applied without effects for cell‐cell and cell‐matrix adhesion.