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Effects of sangre de drago in an in vitro model of cutaneous neurogenic inflammation
Author(s) -
Pereira Ulysse,
GarciaLe Gal Caridad,
Le Gal Grégoire,
Boulais Nicholas,
Lebonvallet Nicolas,
Dorange Germaine,
Lefeuvre Luc,
Gougerot Agnés,
Misery Laurent
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01090.x
Subject(s) - capsaicin , dorsal root ganglion , substance p , in vitro , inflammation , pharmacology , chemistry , croton oil , neurogenic inflammation , medicine , neuropeptide , biochemistry , dorsum , receptor , anatomy
Please cite this paper as: Effects of sangre de drago in an in vitro model of cutaneous neurogenic inflammation. Experimental Dermatology 2010; 19 : 796–799. Abstract: Sangre de drago (SD) is a viscous bright red resin collected from Croton lechleri trees that grow in the South American jungle. This sap is used extensively in the native pharmacopoeia to treat skin disorders. Its effectiveness as an inhibitor of neurogenic inflammation has been recently demonstrated. To understand the underlying mechanisms of these effects, we examined the ability of SD to reduce substance P (SP) release in an in vitro model of cutaneous neurogenic inflammation (CNI). This model is based on an enzyme immunoassay of SP (an inducer of CNI) in a porcine co‐culture of dorsal root ganglion neurons and keratinocytes. After incubation with different concentrations of SD, we noted an immediate and significant dose‐dependent decrease in basal SP release, with average values of 32% at 1% SD (v/v) and 26% at 0.1% (v/v). On the other hand, pretreatment (72 or 1 h) of the co‐culture with 1% SD (v/v) was sufficient to induce a 111% (72 h) or 65% (1 h) inhibition of capsaicin‐induced SP release, while 0.1% SD (v/v) triggered a 109% (72 h) or 30% (1 h) inhibition. We conclude that sangre de drago is a potent inhibitor of CNI through direct inhibition of neuropeptide release by sensory afferent nerves.