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Vitamin C attenuates ERK signalling to inhibit the regulation of collagen production by LL‐37 in human dermal fibroblasts
Author(s) -
Park Hyun Jeong,
Ock Sun Myeong,
Kim Hee Jung,
Park Hong Jin,
Lee Young Bok,
Choi Jung Min,
Cho Chul Soo,
Lee Jun Young,
Cho Baik Kee,
Cho Dae Ho
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01070.x
Subject(s) - mapk/erk pathway , chemistry , kinase , vitamin c , vitamin , phosphorylation , protein kinase a , western blot , p38 mitogen activated protein kinases , keloid , extracellular , signal transduction , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , pathology , gene
Please cite this paper as : Vitamin C attenuates ERK signalling to inhibit the regulation of collagen production by LL‐37 in human dermal fibroblasts. Experimental Dermatology 2010; 19 : e258–e264. Abstract: Vitamin C is used as an anti‐ageing agent because of its collagen enhancing effects. The precise cellular signalling mechanism of vitamin C is not well known. Here, we investigate the profibrotic mechanism of vitamin C against LL‐37. Antimicrobial peptide LL‐37 decreases collagen expression at mRNA and protein levels in human dermal fibroblasts (HDFs). The ability of LL‐37 to inhibit collagen expression is dependent on phosphorylation of extracellular signal‐regulated kinase (ERK). HDFs and human keloid fibroblasts were treated with vitamin C followed by 2 h of LL‐37 treatment. Collagen mRNA expression and total soluble collagen production inhibited by LL‐37 was enhanced by treatment with 0.5 m m vitamin C. Vitamin C also decreased intracellular reactive oxygen intermediates (ROI) levels that were increased by LL‐37. Furthermore, the phosphorylation of ERK was analysed by Western blot following treatment with vitamin C and LL‐37. Vitamin C turned off phosphorylation of ERK that was induced by LL‐37. Ets‐1 transcriptional factor, which is involved in the regulation of collagen expression by LL‐37, was also inhibited by vitamin C. This study shows that vitamin C enhances collagen production by inhibiting the ERK pathway induced by LL‐37.