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Chemokine expression by human keratinocyte cell lines after activation of Toll‐like receptors
Author(s) -
Olaru Florina,
Jensen Liselotte E.
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.01026.x
Subject(s) - hacat , innate immune system , tlr7 , tlr5 , ccl18 , keratinocyte , toll like receptor , chemokine , cxcl10 , microbiology and biotechnology , cell culture , ccl20 , tlr9 , biology , immunology , receptor , imiquimod , immune system , chemokine receptor , gene expression , biochemistry , genetics , dna methylation , gene
Please cite this paper as: Chemokine expression by human keratinocyte cell lines after activation of Toll‐like receptors. Experimental Dermatology 2010; 19 : e314–e316. Abstract: Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll‐like receptor (TLR)‐ligands. Expression of IL‐8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1‐6 and TLR9. KERTr cells responded to the same TLR‐ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL‐8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin.