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Increased expression of MAP2 inhibits melanoma cell proliferation, invasion and tumor growth in vitro and in vivo
Author(s) -
Song Zhiqi,
He ChunDi,
Sun Changkai,
Xu Yanni,
Jin Xin,
Zhang Yi,
Xiao Ting,
Wang Yakun,
Lu Ping,
Jiang Yi,
Wei Huachen,
Chen HongDuo
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.01020.x
Subject(s) - melanoma , in vivo , cancer research , in vitro , cell growth , apoptosis , metastasis , biology , cell culture , cell cycle , cell , pathology , cancer , medicine , biochemistry , genetics , microbiology and biotechnology
Please cite this paper as: Increased expression of MAP2 inhibits melanoma cell proliferation, invasion and tumor growth in vitro and in vivo . Experimental Dermatology 2010; 19 : 958–964. Abstract: Malignant melanoma (MM) is characterized by aggressive metastasis and high mortality rate. Microtubule‐associated proteins 2 (MAP2) is expressed abundantly in majority of melanocytic nevi and primary melanomas, but absent in metastatic melanomas. To determine whether MAP2 correlates with tumor progression of MM, we investigated the effects of MAP2 inhibition on the biological behaviour of metastatic melanoma in vitro and in vivo . Our results demonstrated that adenovirus‐mediated MAP2 induced apoptotic cell death and cell cycle arrest in metastatic human and mouse melanoma cell lines in vitro , and substantially inhibited the growth of melanomas in nude mice in vivo . In addition, intracellular expression of MAP2 was found to induce the morphologic alteration, suppress the migration and invasion and affect the assembly, stabilization and bundling of microtubules in melanoma cells. This is the first study that MAP2 expression significantly inhibits the growth of MM in vivo . Our results suggest that MAP2 may serve as a promising molecular target for therapy and chemoprevention of MM in humans.