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Functional characterization of T cells differentiated in vitro from bone marrow‐derived CD34 + cells of psoriatic patients with family history
Author(s) -
Zhang Kaiming,
Li Xinhua,
Yin Guohua,
Liu Yufeng,
Tang Xuyuan
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.01016.x
Subject(s) - haematopoiesis , bone marrow , cd34 , psoriasis , immunology , stromal cell , stem cell , biology , interleukin 3 , cytokine , progenitor cell , cancer research , interleukin 21 , t cell , microbiology and biotechnology , immune system
Please cite this paper as: Functional characterization of T cells differentiated in vitro from bone marrow‐derived CD34 + cells of psoriatic patients with family history. Experimental Dermatology 2010; 19 : e128–e135. Abstract Background: The strong but complex genetic background suggests that inherent and intrinsic rather than exogenous factors have a key role in immunopathogenesis of psoriasis. It is reasonable to speculate that the dysfunctional activity of psoriatic T cells may partly originate from the abnormal haematopoietic cells. Objectives: To test if T cells originated from haematopoietic progenitor cells in psoriasis patients display functional alternations similar to previously reported abnormalities of circulating T cells. Methods: Bone marrow CD34 + haematopoietic cells were isolated from psoriatic patients with family history and healthy subjects, and differentiated into T cells in vitro in the thymic stromal co‐culture system. These cells were further subjected to functional comparisons such as in vitro proliferation, secretion of cytokines such as IL‐4, IL‐8 and IFN–γ, and inducing the production of C‐myc, Bcl‐xL, and Ki67 proteins in human keratinocytes. Results: While bone marrow‐derived CD34 + cells from both patients and healthy volunteers developed into mature T cells within weeks in the thymic environment in vitro , the differentiated T cells from psoriatic patients showed higher proliferation and stronger capacity to secret TH1 cytokines in response to streptococcal superantigen. The differentiated T cells from psoriatic patients, but not from normal controls, induced overexpression of C‐myc and Ki67, but not Bcl‐XL, in keratinocytes. Conclusions: T cells differentiated from CD34 + cells of psoriatic patients, but not normal controls, are functionally similar to psoriatic circulating T cells, suggesting that the dysfunctional activity of T cells in psoriatic patients can be traced back to the early development of haematopoietic cells.