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Upregulation of cathepsin S in psoriatic keratinocytes
Author(s) -
Schönefuß Alexander,
Wendt Wiebke,
Schattling Benjamin,
Schulten Roxane,
Hoffmann Klaus,
Stuecker Markus,
Tigges Christian,
Lübbert Hermann,
Stichel Christine
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00990.x
Subject(s) - dermis , psoriasis , cats , downregulation and upregulation , pathology , immunostaining , mhc class i , biology , immunology , keratinocyte , medicine , cancer research , major histocompatibility complex , immunohistochemistry , antigen , cell culture , biochemistry , gene , genetics
Please cite this paper as: Upregulation of cathepsin S in psoriatic keratinocytes. Experimental Dermatology 2010; 19 : e80–e88. Abstract: Cathepsin S (CATS) is a cysteine protease, well known for its role in MHC class II‐mediated antigen presentation and extracellular matrix degradation. Disturbance of the expression or metabolism of this protease is a concomitant feature of several diseases. Given this importance we studied the localization and regulation of CATS expression in normal and pathological human/mouse skin. In normal human skin CATS‐immunostaining is mainly present in the dermis and is localized in macrophages, Langerhans, T‐ and endothelial cells, but absent in keratinocytes. In all analyzed pathological skin biopsies, i.e. atopic dermatitis, actinic keratosis and psoriasis, CATS staining is strongly increased in the dermis. But only in psoriasis, CATS‐immunostaining is also detectable in keratinocytes. We show that cocultivation with T‐cells as well as treatment with cytokines can trigger expression and secretion of CATS, which is involved in MHC II processing in keratinocytes. Our data provide first evidence that CATS expression (i) is selectively induced in psoriatic keratinocytes, (ii) is triggered by T‐cells and (iii) might be involved in keratinocytic MHC class II expression, the processing of the MHC class II‐associated invariant chain and remodeling of the extracellular matrix. This paper expands our knowledge on the important role of keratinocytes in dermatological disease.