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Signal transducer and activator of transcription 3 upregulates interleukin‐8 expression at the level of transcription in human melanoma cells
Author(s) -
Oka Masahiro,
Sakaguchi Masanobu,
Okada Taro,
Nagai Hiroshi,
Ozaki Michitaka,
Yoshioka Toyo,
Inoue Hiroshi,
Mukaida Naofumi,
Kikkawa Ushio,
Nishigori Chikako
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00972.x
Subject(s) - stat3 , stat protein , transfection , microbiology and biotechnology , transcription (linguistics) , melanoma , reporter gene , gene knockdown , cancer research , stat4 , cell culture , activator (genetics) , transcription factor , biology , chemistry , gene expression , phosphorylation , stat , gene , biochemistry , linguistics , philosophy , genetics
Please cite this paper as: Signal transducer and activator of transcription 3 upregulates interleukin‐8 expression at the level of transcription in human melanoma cells. Experimental Dermatology 2010; 19 : e50–e55. Abstract: Many melanoma cells continuously produce interleukin‐8 (IL‐8). The involvement of signal transducer and activator of transcription 3 (STAT3) in the constant production of IL‐8 in melanoma cells was examined. The level of IL‐8 production correlated well with that of the phosphorylated (activated) STAT3 in six human melanoma cell lines. Introduction of the constitutively activated form of STAT3 (STAT3‐C) into WM35 melanoma cells, that show low levels of IL‐8 and phosphorylated STAT3, enhanced IL‐8 production. Knockdown of STAT3 suppressed IL‐8 production in WM1205Lu cells that contain a high level of IL‐8 accompanied by STAT3 phosphorylation. Introduction of STAT3‐C markedly increased the luciferase activity in WM1205Lu cells transfected with reporter vectors linked to the 5′‐flanking region of the IL‐8 gene from ‐546 to +44 base pair (bp) and from ‐272 to +44 bp, but not in cells expressing reporter plasmids from ‐133 to +44 bp and from ‐98 to +44 bp. These results indicate that the upregulation of IL‐8 production is caused by constitutive STAT3 activation at the level of gene transcription in melanoma cells.